Hematology Tests and Protocols

Hematology Tests

Research,
Hematology Testing
Test Description
Performed on the Advia 2120
Specimen Minimum
Volume
Required*
Species
*Customized pricing may apply for high volume testing, batch testing or panels of tests. Contact the Clinical Pathology Lab for additional information regarding discount pricing.  
EDTA
CBC, Complete Blood Count Includes White Blood Count, Red Blood Count and indices, and Platelet count. Automated reticulocyte counts included for cats and dogs. Automated differentials that meet lab specified criteria are confirmed by manual examination of slides by lab staff. Slides are examined for cell morphology, parasites and abnormal cells. Additional review of slides is available by Board Certified Clinical Pathologists for slides that meet lab specified criteria or study requirements. EDTA 400 ul Bovine, Camelids, Canine, Feline, Mice, Rats, Rabbits, Equine, Porcine, Non-human Primates, Ovine and others (contact lab)
Blood Smear Review EDTA or slides are examined for parasites, abnormal cells or cell morphology. Test order must include description of item for review. One request item/order. For abnormal cells or cell morphology a CBC is recommended. Additional review of slides is available by Board Certified Clinical Pathologists for slides that meet lab specified criteria or study requirements.  EDTA or Slides (minimum 2)  400 ul  Bovine, Camelids, Canine, Feline, Mice, Rats, Rabbits, Equine, Porcine, Non-human Primates, Ovine and others (contact lab)
Cell Count Only White Blood Count, Red Cell Count and indices, Platelet count. (Differential is NOT included in this test. See CBC if differential is required) Manual review of slide is NOT included in this test.  EDTA  250 ul  Bovine, Camelids, Canine, Feline, Mice, Rats, Rabbits, Equine, Porcine, Non-human Primates, Ovine and others (contact lab)
Buffy Coat Smears  Blood smears prepared from buffy coat are examined for parasites or abnormal cells. One request item per order.  EDTA or Slides (minimum 2)  250 ul  Bovine, Camelids, Canine, Feline, Mice, Rats, Rabbits, Equine, Porcine, Non-human Primates, Ovine and others (contact lab)
Platelet Count  Automated platelet count  EDTA  400 ul  Bovine, Camelids, Canine, Feline, Mice, Rats, Rabbits, Equine, Porcine, Non-human Primates, Ovine and others (contact lab)
Reticulocyte count  Automated reticulocyte counts available for cats and dogs.   EDTA  400 ul  Canine, Felines and Non-human Primates.
*Automated CBC testing can be performed with a minimum volume of 200 uL of EDTA whole blood. Volumes of less than 200 uL cannot be performed using automated methods. Contact lab for additional options.

Small Volume Protocols

Collection protocol for small volume specimens (mice, rabbits, etc.) 

Instruments

Advia 2120 Hematology Analyzer, version 2.00.00, multi-species; Siemens Healthcare Diagnostics Inc., Deerfield, IL

Sample Requirements

EDTA whole blood (lavender top tube); minimum volume of 400 μLs is required for sampling processing; tubes of appropriate size should be selected to ensure an optimal ratio of EDTA to whole blood.

 
 Option  Description of item

Required EDTA, Whole Blood volume

 1 CBC- includes RBC count, Hgb, Hct, MCV, MCH, MCHC, RDW, plt count, MPV, PCT, PDW, PCV, total plasma protein, plasma color, manual WBC 100 cell differential count and evaluation of RBC morphologic changes  400 μL*
 2 Mini-CBC (same as above, but without manual differential cell count and RBC morphology)  400 μL*
 3 Platelet count and fibrinogen  500 μL*

*In some instances, automated CBC testing can be performed with a minimum volume of 200 uL of EDTA whole blood. Volumes of less than 200 uL cannot be performed using automated methods. Contact lab for additional options.

Appropriate Tubes

The following tubes are suitable for blood collection and analysis using the Advia analyzer for animals in which only small volumes of blood can be collected safely.  Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.

 Name Sample volume Mfr #
BD EDTA Microtainer Capillary Blood Collector  500 uL  365973
BD EDTA Microtainer Capillary Blood Collector with BD Microgard Closure  500 uL  365974
Greiner EDTA Vacuette MiniCollect Capilary Blood Collection System (requires purchase of specific capillary tube)  500 uL  450404

Sample Handling

Following collection, tubes should be inverted 8 times to ensure adequate mixing of blood and EDTA.  Label tubesusing permanent marker with a legible, simplified numbering system.  Samples should be refrigerated as soon as possible following collection.  They should not be frozen or transported directly on ice.  Transport with cold packs is recommended.  

Results

CBC parameters generated from the Advia include RBC count, hemoglobin, hematocrit, MCV, MCH, MCHC, RDW, platelet count, MPV, PCT and PDW.  Results generated by laboratory personnel include PCV, total plasma protein, plasma color (if abnormal), WBC and platelet estimate, 100 cell differential count, and RBC morphologic changes.  Any abnormal results are reviewed by the clinical pathologist on duty.  Results are available in Excel format.  Slides and data are stored for 2 years in the laboratory, unless specially arranged with the lab.

Common Interferences

Samples with clots cannot be analyzed due to inaccurate results and the potential to damage laboratory equipment.  Hemolyzed and lipemic samples may artifactually elevate or invalidate the total plasma protein.  Hemolysis may invalidate the hemogloblin concentration, MCH and MCHC calculations.

Large Volume Protocols

Collection protocol for large volume specimens (dogs, horses, etc.)

Instruments

Advia 2120 Hematology Analyzer, version 2.00.00, multi-species; Siemens Healthcare Diagnostics Inc., Deerfield, IL

Sample Requirements

EDTA whole blood (lavender top tube); minimum volume of 400 μLs is required for sampling processing; tubes of appropriate size should be selected to ensure an optimal ratio of EDTA to whole blood.

 

 Option  Description of item

Required EDTA, Whole Blood volume

 1 CBC- includes RBC count, Hgb, Hct, MCV, MCH, MCHC, RDW, plt count, MPV, PCT, PDW, PCV, total plasma protein, plasma color, manual WBC 100 cell differential count and evaluation of RBC morphologic changes  400 μL*
 2 Mini-CBC (same as above, but without manual differential cell count and RBC morphology)  400 μL*
 3 Platelet count and fibrinogen (alone or as part of the coagulation profile)  500 μL*

**In some instances, automated CBC testing can be performed with a minimum volume of 200 uL of EDTA whole blood. Volumes of less than 200 uL cannot be performed using automated methods. Contact lab for additional options. Small volume samples should be collected into tubes designed for smaller volumes of blood (see the form, Complete Blood Count (CBC): Collection Protocols for Small Volume Specimens - Mice, Rats, and Rabbits, for appropriate collection tubes).  Contact lab for additional options.

Appropriate Tubes

The following tubes are suitable for blood collection and analysis using the Advia analyzer for animals in which only small volumes of blood can be collected safely.  Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.

 Name Sample volume Mfr #
BD Vacutainer Blood Tubes with K2EDTA  2 mL  367841
BD Vacutainer Blood Tubes with K2EDTA  3 mL  367856


Sample Handling

Following collection, tubes should be inverted 8 times to ensure adequate mixing of blood and EDTA.  Label tubesusing permanent marker with a legible, simplified numbering system.  Samples should be refrigerated as soon as possible following collection.  They should not be frozen or transported directly on ice.  Transport with cold packs is recommended. 

Results

CBC parameters generated from the Advia include RBC count, hemoglobin, hematocrit, MCV, MCH, MCHC, RDW, platelet count, MPV, PCT and PDW.  Results generated by laboratory personnel include PCV, total plasma protein, plasma color (if abnormal), WBC and platelet estimate, 100 cell differential count, and RBC morphologic changes.  Any abnormal results are reviewed by the clinical pathologist on duty.  Results are available in Excel format.  Slides and data are stored for 2 years in the laboratory, unless specially arranged with the lab.

Common Interferences

Samples with clots cannot be analyzed due to inaccurate results and the potential to damage laboratory equipment.  Hemolyzed and lipemic samples may artifactually elevate or invalidate the total plasma protein.  Hemolysis may invalidate the hemogloblin concentration, MCH and MCHC calculations.