Chemistry Tests and Protocols

 

Chemistry Single Tests

Serum

Test name Test initials Specimen Minimum volume
Alanine Aminotransferase ALT Serum 50 ul
Albumin Alb Serum 50 ul
Alkaline Phosphatase ALP Serum 50 ul
Amylase Amy Serum 50 ul
Aspartate Aminotransferase AST Serum 50 ul
Beta-hydroxybutyrate BHY Serum 50 ul
Bicarbonate HCO3 Serum 50 ul
Bile acid (Fasting & Post Prandial) Bile Serum 50 ul
Bilirubin, Total Tbili Serum 50 ul
Blood Urea Nitrogen BUN Serum 50 ul
Calcium Ca Serum 50 ul
Chloride Cl Serum 50 ul
Cholesterol Chol Serum 50 ul
Creatine Kinase CK Serum 50 ul
Creatinine Creat Serum 50 ul
Gamma Glutamyltransferase GGT Serum 50 ul
Glucose Gluc Serum 50 ul
Lactate Dehydrogenase LDH Serum 50 ul
Magnesium Mg Serum 50 ul
Phenobarbitol Pheno Serum 50 ul
Phosphorous Phos Serum 50 ul
Potassium K Serum 50 ul
Sodium Na Serum 50 ul
Sorbitol Dehydrogenase SDH Serum 50 ul
Total Protein TP Serum 50 ul
Triglyceride Trig Serum 50 ul


CSF

Test name Test initials Speciman Minimum Volume
CSF Creatine Kinase CKCSF Spinal Fluid 50 ul
CSF Glucose GluCSF Spinal Fluid 50 ul
CSF Total Protein MTPCSF Spinal Fluid 50 ul
CSF CK & Protein   Spinal Fluid 75 ul
CSF Protein, CK and Glucose   Spinal Fluid 75 ul


Urine

Test name Test initials Speciman Minimum Volume
Urine Calcium CaU Urine 50 ul
Urine Chloride ClU Urine 50 ul
Urine Creatinine CreatU Urine 50 ul
Urine Chloride/Creatinine Cl/CreatU Urine 75 ul
Urine Gamma Glutamyltransferase GGTU Urine 50 ul
Urine Magnesium MgU Urine 50 ul
Urea Nitrogen Urea Urine 50 ul
Urine Phosphorous PhosU Urine 50 ul
Urine Potassium KU Urine 50 ul
Urinary Protein MTPU Urine 50 ul
Urine Protein/Creatinine Ratio UP/UC Urine 50 ul
Urine Sodium NaU Urine 50 ul

Chemistry Panels

Research Panels Available Tests included in panels Minimum Volume Species
Electrolyte Profile Anion Gap, HCO3, Ca, Cl, Mg, Phos, Na, K, LIH 250 ul Alpacas, Bovine, Camelids, Canines, Equine, Feline, Llamas, Mice, Mink, Porcine, Rabiits, Rats, Other
Large Animal Hepatic Alb, ALP, AST, ALT, CK, GGT, SDH, TP, Gluc, Tbili, LIH 250 ul Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Large Animal Mini-Basic HCO3, Ca, Cl, CK, GGT, Gluc, K, TP, Creat, Na, LIH 250 ul Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Large Animal Profile Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, CK, Creat, GGT, Gluc, Glob, LIH, MG, Osmolality, Phos, K, Na, SDH, TP 250 ul Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Non-human Primate ALT, Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, Chol, CK, Creat, GGT, Globulin, Gluc, LIH, Mg, Osmolality, K, Na, TP, Trig 250 ul Non-human Primates
Pick 2 Research Panel Pick any two analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids) 50 ul Serum Samples ONLY. Available for most lab species. Check with lab for exotic species.
Pick 5 Research Panel Pick any five analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids) 100 ul Serum Samples ONLY. Available for most lab species. Check with lab for exotic species.
Renal Profile Alb, Anion Gap, BUN, Ca, Chol, Cl, Creat, HCO3, Gluc,K, Mg, Na, Osmolality,Total Protein, BHY, Phos 250 ul Available for routinely utilized lab species. Check with lab for exotic species.
Small Animal Hepatic ALT, Alb, ALP, Amylase, AST. Tbili, Chol, GGT, TP 250 ul Canine, Feline, Mice, Mink, Rabbits, Rats
Small Animal Profile ALT, Alb, ALP, Amylase, Anion Gap, AST, HCO3, BUN, Ca, Cl, Chol, Creat, GGT Gluc,Globulin, LIH, Mg, Osmolality, Phos, K, Na, TP, Tbili, CK 250 ul Canine, Feline, Mice, Mink, Rabbits, Rats
Small Animal, Basic ALT, Alb, ALP, Tbili, BUN, Creat, Gluc, K, Na, TP, LIH, Anion Gap, C1, HCO3 250 ul Canine, Feline, Mice, Mink, Rabbits, Rats
Urine Profile Cl, Creat, K, Na 150 ul Most species. Check with lab for exotic species.

Small Volume Protocols

Small volume biochemical profile: Collection protocols for small volume specimens (mice, rats, rabbits, etc.)

Instrument

Beckman Coulter AU480; Beckman Coulter, Brea, CA

Sample Requirements

Blood should be collected into plain red top tubes with no additive or gel separator.  Following collection, blood should be allowed to clot, centrifuged at 1700 rcf* for 10 minutes, the serum removed and placed into another plain red top tube or other, sterile, additive-free, plastic tube.  To prevent analytical error, serum should be removed from the clot within 1 hour of sampling.  Required volumes are listed in the table below.   

*RCF = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2

 Option  Description of item

Required serum volume
(Note: not whole blood volume)

 1 Single test 50 μLs**
 2 Two tests 75 μLs**
 3 Research panel of up to 5 tests (can be customized) 100 μLs**
 4 Larger panels and profiles 250 μLs**

 **If the specimen is short volume, the lab will attempt to run as many tests as possible.

Appropriate Tubes

The following tubes are suitable for blood collection and analysis using the Beckman AU480.  Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.

 Name Sample volume Mfr #
 BD Microtainer Capillary Blood Collector  400 uL  365957
 BD Microtainer Capillary Blood Collector with BD Microgard Closure  250-500 uL  365963

Sample Handling

Label tubes using permanent marker with a legible, simplified numbering system.  Samples should be refrigerated as soon as possible following collection.  Transport with cold packs is recommended; do not transport directly on ice.  Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis. 

Results

Results are available in Excel format.

Common Interferences

Certain degrees of lipemia, hemolysis, and icterus (LIH) may interfere with analysis of some analytes.  LIH indices and the expected interferences are reported for basic and full profile samples. Potassium levels may be artifactually increased and glucose artifactually decreased in samples where serum has sat on the blood clot for an extended period of time.

Large Volume Protocols

Large volume biochemical profile: Collection protocols for large volume specimens (dogs, pigs, camelids, horses, etc.)

Instrument

Beckman AU480, Beckman Coulter, Brea, CA

Sample Requirements

Blood should be collected into plain red top tubes with no additive or gel separator.  Following collection, blood should be allowed to clot, centrifuged at 1700 rcf* for 10 minutes, the serum removed and placed into another plain red top tube or other, sterile, additive-free, plastic tube.  To prevent analytical error, serum should be removed from the clot within 1 hour of sampling.  Required volumes are listed in the table below.   

*RCF = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2

 Option  Description of item

Required serum volume
(Note: not whole blood volume)

 1 Single test 50 μLs**
 2 Two tests 75 μLs**
 3 Research panel of up to 5 tests (can be customized) 100 μLs**
 4 Larger panels and profiles 250 μLs**

 **If the specimen is short volume, the lab will attempt to run as many tests as possible.

Appropriate Tubes

The following tubes are suitable for blood collection and analysis using the Beckman Coulter AU480.  Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.

 Name Sample volume Mfr #
BD Vacutainer Blood Tubes (glass tubes, no additive)  3 mL  366397
BD Vacutainer Blood Tubes (glass tubes, no additive)  7 mL  366397
BD Vacutainer Blood Tubes (glass tubes, no additive)  10 mL  

Sample Handling

Label tubes using permanent marker with a legible, simplified numbering system.  Samples should be refrigerated as soon as possible following collection.  Transport with cold packs is recommended; do not transport directly on ice.  Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis. 

Results

Results are available in Excel format.

Common Interferences

Certain degrees of lipemia, hemolysis, and icterus (LIH) may interfere with analysis of some analytes.  LIH indices and the expected interferences are reported for basic and full profile samples. Potassium levels may be artifactually increased and glucose artifactually decreased in samples where serum has sat on the blood clot for an extended period of time.